› 8g1i

Electron Microscopy
3.12Å resolution

SpCas9 with sgRNA and target DNA

Released:
DOI: 10.2210/pdb8g1i/pdb
PDB entry 8g1i coloured by chain, front view.
PDB entry 8g1i coloured by chain, side view.
PDB entry 8g1i coloured by chain, top view.
Source organisms:
Entry authors: Korolev S, Gagnon K
Related structures: EMD-29671

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-215679 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1384 amino acids
Theoretical weight: 160.77 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
DNA E2_TS Chain: C
Molecule details ›
Chain: C
Length: 40 nucleotides
Theoretical weight: 12.33 KDa
Source organism: Renilla reniformis
Expression system: Not provided
DNA E2_NTS Chain: D
Molecule details ›
Chain: D
Length: 40 nucleotides
Theoretical weight: 12.29 KDa
Source organism: Renilla reniformis
Expression system: Not provided
sgRNA Chain: B
Molecule details ›
Chain: B
Length: 98 nucleotides
Theoretical weight: 31.54 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.12Å
Relevant EMDB volumes: EMD-29671
Expression systems:
  • Escherichia coli
  • Not provided

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