› 8g1s

Electron Microscopy
3.7Å resolution

Cryo-EM structure of 3DVA component 1 of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase minus preQ1 ligand

Released:
DOI: 10.2210/pdb8g1s/pdb
PDB entry 8g1s coloured by chain, front view.
PDB entry 8g1s coloured by chain, side view.
PDB entry 8g1s coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for control of bacterial RNA polymerase pausing by a riboswitch and its ligand.
Chauvier A, Porta JC, Deb I, Ellinger E, Meze K, Frank AT, Ohi MD, Walter NG
Nat Struct Mol Biol (2023)
PMID: 37264140
Related structures: EMD-29676

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235639 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (7 distinct):
DNA-directed RNA polymerase subunit alpha Chains: G, H
Molecule details ›
Chains: G, H
Length: 235 amino acids
Theoretical weight: 26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-235; Coverage: 71%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: I
Molecule details ›
Chain: I
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: J
DNA-directed RNA polymerase subunit omega Chain: K
DNA (39-MER) Chain: A
DNA (31-MER) Chain: B
RNA (47-MER) Chain: R

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.7Å
Relevant EMDB volumes: EMD-29676
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Riboswitches as therapeutic targets: promise of a new era of antibiotics.
Ellinger et al. (2023)
2 more

1 mention without citation

Structural Insights into Protein-Aptamer Recognitions Emerged from Experimental and Computational Studies.
Troisi et al. (2023)