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Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins.

Kubo S, Black CS, Joachimiak E, Yang SK, Legal T, Peri K, Khalifa AAZ, Ghanaeian A, McCafferty CL, Valente-Paterno M, De Bellis C, Huynh PM, Fan Z, Marcotte EM, Wloga D, Bui KH
Nat Commun 14 2168 (2023)
Cited: 7 times
EuropePMC logo PMID: 37061538

Abstract

Cilia are ubiquitous eukaryotic organelles responsible for cellular motility and sensory functions. The ciliary axoneme is a microtubule-based cytoskeleton consisting of two central singlets and nine outer doublet microtubules. Cryo-electron microscopy-based studies have revealed a complex network inside the lumen of both tubules composed of microtubule-inner proteins (MIPs). However, the functions of most MIPs remain unknown. Here, we present single-particle cryo-EM-based analyses of the Tetrahymena thermophila native doublet microtubule and identify 42 MIPs. These data shed light on the evolutionarily conserved and diversified roles of MIPs. In addition, we identified MIPs potentially responsible for the assembly and stability of the doublet outer junction. Knockout of the evolutionarily conserved outer junction component CFAP77 moderately diminishes Tetrahymena swimming speed and beat frequency, indicating the important role of CFAP77 and outer junction stability in cilia beating generation and/or regulation.

Reviews citing this publication (1)

  1. Lumenal components of cytoplasmic microtubules. Tsuji C, Dodding MP. Biochem Soc Trans 50 1953-1962 (2022)

Articles citing this publication (6)

  1. A Comprehensive Genetic Study of Microtubule-Associated Gene Clusters for Male Infertility in a Taiwanese Cohort. Chan CC, Yen TH, Tseng HC, Mai B, Ho PK, Chou JL, Wu GJ, Huang YC. Int J Mol Sci 24 15363 (2023)
  2. CEP104/FAP256 and associated cap complex maintain stability of the ciliary tip. Legal T, Parra M, Tong M, Black CS, Joachimiak E, Valente-Paterno M, Lechtreck K, Gaertig J, Bui KH. J Cell Biol 222 e202301129 (2023)
  3. Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes. Wang Y, Yang J, Hu F, Yang Y, Huang K, Zhang K. J Cell Biol 222 e202301066 (2023)
  4. FAP106 is an interaction hub for assembling microtubule inner proteins at the cilium inner junction. Shimogawa MM, Wijono AS, Wang H, Zhang J, Sha J, Szombathy N, Vadakkan S, Pelayo P, Jonnalagadda K, Wohlschlegel J, Zhou ZH, Hill KL. Nat Commun 14 5225 (2023)
  5. Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism. Ghanaeian A, Majhi S, McCafferty CL, Nami B, Black CS, Yang SK, Legal T, Papoulas O, Janowska M, Valente-Paterno M, Marcotte EM, Wloga D, Bui KH. Nat Commun 14 5741 (2023)
  6. Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium. Erickson T, Biggers WP, Williams K, Butland SE, Venuto A. J Dev Biol 11 28 (2023)


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