› 8g3k

Electron Microscopy
2.2Å resolution

Cryo-EM imaging scaffold subunits A and B used to display KRAS G12C complex with GDP

Released:
DOI: 10.2210/pdb8g3k/pdb
PDB entry 8g3k coloured by chain, front view.
PDB entry 8g3k coloured by chain, side view.
PDB entry 8g3k coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure determination of small therapeutic protein targets at 3 Å-resolution using a rigid imaging scaffold.
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JÉ, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
OpenAccess logo Proc Natl Acad Sci U S A 120 e2305494120 (2023)
PMID: 37669364
Related structures: EMD-29700

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-271753 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cobalamin adenosyltransferase-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 20.17 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: O58404 (Residues: 1-172; Coverage: 100%)
Gene name: PH0671
Sequence domains: Cobalamin adenosyltransferase
Cryo-EM imaging scaffold subunit B with DARPin - RCG-33 Chain: B
Molecule details ›
Chain: B
Length: 321 amino acids
Theoretical weight: 35.45 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.2Å
Relevant EMDB volumes: EMD-29700
Expression system: Escherichia coli

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Citations

1 review citation

Applications and prospects of cryo-EM in drug discovery.
Zhu et al. (2023)
1 other article