› 8g4e

Electron Microscopy
2.98Å resolution

Green Fluorescence Protein imaged on a cryo-EM imaging scaffold

Released:
DOI: 10.2210/pdb8g4e/pdb
PDB entry 8g4e coloured by chain, front view.
PDB entry 8g4e coloured by chain, side view.
PDB entry 8g4e coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure determination of small therapeutic protein targets at 3 Å-resolution using a rigid imaging scaffold.
Castells-Graells R, Meador K, Arbing MA, Sawaya MR, Gee M, Cascio D, Gleave E, Debreczeni JÉ, Breed J, Leopold K, Patel A, Jahagirdar D, Lyons B, Subramaniam S, Phillips C, Yeates TO
OpenAccess logo Proc Natl Acad Sci U S A 120 e2305494120 (2023)
PMID: 37669364
Related structures: EMD-29718

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239126 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RCG-10 - Cryo-EM imaging scaffold subunit B fused to DARPin Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 35.29 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Green fluorescent protein Chain: B
Molecule details ›
Chain: B
Length: 234 amino acids
Theoretical weight: 26.62 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-231; Coverage: 97%)
Gene name: GFP
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
Resolution: 2.98Å
Relevant EMDB volumes: EMD-29718
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Applications and prospects of cryo-EM in drug discovery.
Zhu et al. (2023)
1 other article