› 8g4p

X-ray diffraction
2.25Å resolution

Crystal structure of the peanut allergen Ara h 2 bound by two neutralizing antibodies 13T1 and 13T5

Released:
DOI: 10.2210/pdb8g4p/pdb
Model geometry
Fit model/data
PDB entry 8g4p coloured by chain, front view.
PDB entry 8g4p coloured by chain, side view.
PDB entry 8g4p coloured by chain, top view.
Source organisms:
Primary publication:
Design of an Ara h 2 hypoallergen from conformational epitopes.
Min J, Keswani T, LaHood NA, Lytle IR, Marini-Rapoport O, Andrieux L, Sneed SL, Edwards LL, Petrovich RM, Perera L, Pomés A, Pedersen LC, Patil SU, Mueller GA
Clin Exp Allergy (2024)
PMID: 38168500

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-275706 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
13t1 Fab heavy chain Chain: C
Molecule details ›
Chain: C
Length: 231 amino acids
Theoretical weight: 24.48 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
13T1 Fab light chain Chain: D
Molecule details ›
Chain: D
Length: 216 amino acids
Theoretical weight: 23.67 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
13T5 Fab heavy chain Chain: A
13T5 Fab light chain Chain: B
Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein Chain: E

Ligands and Environments

4 bound ligands:
Ligand EDO 6 x EDO
Ligand CL 7 x CL
Ligand SO4 4 x SO4
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 136.302Å b: 136.794Å c: 96.819Å
α: 90° β: 127.34° γ: 90°
R-values:
R R work R free
0.19 0.189 0.218
Expression systems:
  • Cricetulus griseus
  • Escherichia coli

Quick links

Citations

1 review citation

The Potential of Human Monoclonal IgE Antibodies to Establish Biological Potency and Stability of Allergen Extracts.
Rabin RL. (2024)
1 other article

1 mention without citation

Origins and diversity of pan-isotype human bone marrow plasma cells.
Pacheco et al. (2024)