› 8g6e

Electron Microscopy
2.18Å resolution

Structure of the Plasmodium falciparum 20S proteasome complexed with inhibitor TDI-8304

Released:
DOI: 10.2210/pdb8g6e/pdb
PDB entry 8g6e coloured by chain, front view.
PDB entry 8g6e coloured by chain, side view.
PDB entry 8g6e coloured by chain, top view.
Source organism: Plasmodium falciparum NF54
Primary publication:
Structures revealing mechanisms of resistance and collateral sensitivity of Plasmodium falciparum to proteasome inhibitors.
Hsu HC, Li D, Zhan W, Ye J, Liu YJ, Leung A, Qin J, Crespo B, Gamo FJ, Zhang H, Cui L, Roth A, Kirkman LA, Li H, Lin G
OpenAccess logo Nat Commun 14 8302 (2023)
PMID: 38097652
Related structures: EMD-29764

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236765 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Proteasome alpha-type subunits domain-containing protein Chains: A, O
Molecule details ›
Chains: A, O
Length: 260 amino acids
Theoretical weight: 29.53 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: W7JVP8 (Residues: 1-260; Coverage: 100%)
Gene names: CK202_2446, CYL21_1296, PFNF54_02078
Sequence domains:
Proteasome alpha-type subunits domain-containing protein Chains: B, P
Molecule details ›
Chains: B, P
Length: 235 amino acids
Theoretical weight: 26.56 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: A0A2I0BQ13 (Residues: 1-235; Coverage: 100%)
Gene names: CK202_4966, CYL21_4378
Sequence domains:
Proteasome subunit alpha type Chains: C, Q
Molecule details ›
Chains: C, Q
Length: 246 amino acids
Theoretical weight: 27.98 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: W7KN95 (Residues: 1-246; Coverage: 100%)
Gene names: CK202_4303, CYL21_5399, PFNF54_00272
Sequence domains:
Proteasome subunit alpha type Chains: D, R
Molecule details ›
Chains: D, R
Length: 241 amino acids
Theoretical weight: 27.26 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: A0A2I0BS43 (Residues: 1-241; Coverage: 100%)
Gene names: CK202_4499, CYL21_5482
Sequence domains:
Proteasome subunit alpha type Chains: E, S
Molecule details ›
Chains: E, S
Length: 256 amino acids
Theoretical weight: 28.42 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: A0A2I0BP34 (Residues: 1-256; Coverage: 100%)
Gene names: CK202_5367, CYL21_5183
Sequence domains:
Proteasome alpha-type subunits domain-containing protein Chains: F, T
Molecule details ›
Chains: F, T
Length: 253 amino acids
Theoretical weight: 28.74 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: W7K5W7 (Residues: 1-253; Coverage: 100%)
Gene names: CK202_0007, CYL21_2454, PFNF54_05872
Sequence domains:
Proteasome alpha-type subunits domain-containing protein Chains: G, U
Molecule details ›
Chains: G, U
Length: 252 amino acids
Theoretical weight: 29.32 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: W7K040 (Residues: 1-252; Coverage: 100%)
Gene names: CK202_3814, CYL21_3097, PFNF54_00518
Sequence domains:
Proteasome subunit beta type-6 Chains: H, V
Molecule details ›
Chains: H, V
Length: 252 amino acids
Theoretical weight: 29.14 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: W7JUG8 (Residues: 31-282; Coverage: 89%)
Gene names: CK202_4201, CYL21_1069, PFNF54_02629
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: I, W
Molecule details ›
Chains: I, W
Length: 229 amino acids
Theoretical weight: 25.1 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: W7K1J4 (Residues: 42-270; Coverage: 85%)
Gene names: CK202_2690, CYL21_4000, PFNF54_04378
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: J, X
Molecule details ›
Chains: J, X
Length: 218 amino acids
Theoretical weight: 24.53 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: A0A2I0BXS0 (Residues: 1-218; Coverage: 100%)
Gene names: CK202_2185, CYL21_3554
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: K, Y
Molecule details ›
Chains: K, Y
Length: 195 amino acids
Theoretical weight: 22.89 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: W7JKG5 (Residues: 1-195; Coverage: 100%)
Gene names: CK202_0043, CYL21_2079, PFNF54_05825
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: L, Z
Molecule details ›
Chains: L, Z
Length: 211 amino acids
Theoretical weight: 23.62 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: W7K6A8 (Residues: 61-271; Coverage: 78%)
Gene names: CK202_4752, CYL21_3663, PFNF54_02809
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: M, a
Molecule details ›
Chains: M, a
Length: 240 amino acids
Theoretical weight: 27.3 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: A0A2I0BU46 (Residues: 1-240; Coverage: 100%)
Gene names: CK202_3362, CYL21_3125
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: N, b
Molecule details ›
Chains: N, b
Length: 265 amino acids
Theoretical weight: 30.91 KDa
Source organism: Plasmodium falciparum NF54
UniProt:
  • Canonical: W7K6I2 (Residues: 1-265; Coverage: 100%)
Gene names: CK202_2268, CYL21_1305, PFNF54_02044
Sequence domains: Proteasome subunit

Ligands and Environments

1 bound ligand:
Ligand YRE 6 x YRE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.18Å
Relevant EMDB volumes: EMD-29764

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