› 8g74

Electron Microscopy
2.5Å resolution

SARS-CoV-2 spike/Nb3 complex with 1 RBD up and 2 Nb3

Released:
DOI: 10.2210/pdb8g74/pdb
PDB entry 8g74 coloured by chain, front view.
PDB entry 8g74 coloured by chain, side view.
PDB entry 8g74 coloured by chain, top view.
Source organisms:
Primary publication:
Discovery of Nanosota-2, -3, and -4 as super potent and broad-spectrum therapeutic nanobody candidates against COVID-19.
Ye G, Pan R, Bu F, Zheng J, Mendoza A, Wen W, Du L, Spiller B, Wadzinski BE, Liu B, Perlman S, Li F
J Virol 97 e0144823 (2023)
PMID: 37855638
Related structures: EMD-29796

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273012 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Nanosota-3 Chains: C, E
Molecule details ›
Chains: C, E
Length: 136 amino acids
Theoretical weight: 14.89 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli
Spike glycoprotein Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 1234 amino acids
Theoretical weight: 136.83 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DTC2 (Residues: 14-1211; Coverage: 95%)
Gene names: 2, S
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 25 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.5Å
Relevant EMDB volumes: EMD-29796
Expression systems:
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

2 review citations

Therapeutic nanobodies against SARS-CoV-2 and other pathogenic human coronaviruses.
Yang et al. (2024)
1 more