› 8g9z

X-ray diffraction
2.07Å resolution

High-resolution crystal structure of the human selenomethionine-derived SepSecS-tRNASec complex

Released:
DOI: 10.2210/pdb8g9z/pdb
Model geometry
Fit model/data
PDB entry 8g9z coloured by chain, front view.
PDB entry 8g9z coloured by chain, side view.
PDB entry 8g9z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the tRNA-dependent activation of the terminal complex of selenocysteine synthesis in humans.
Puppala AK, Castillo Suchkou J, French RL, Kiernan KA, Simonović M
OpenAccess logo Nucleic Acids Res (2023)
PMID: 36929010

Function and Biology Details

Reaction catalysed: 
O-phospho-L-seryl-tRNA(Sec) + selenophosphate + H(2)O = L-selenocysteinyl-tRNA(Sec) + 2 phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-290189 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
O-phosphoseryl-tRNA(Sec) selenium transferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 521 amino acids
Theoretical weight: 58.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HD40 (Residues: 1-501; Coverage: 100%)
Gene names: SEPSECS, TRNP48
Sequence domains: O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS
RNA (90-MER) Chain: E
Molecule details ›
Chain: E
Length: 90 nucleotides
Theoretical weight: 28.91 KDa
Source organism: Homo sapiens
Expression system: Not provided
Sequence domains: Selenocysteine transfer RNA

Ligands and Environments


Cofactor: Ligand PLR 4 x PLR
1 bound ligand:
Ligand MPD 4 x MPD
1 modified residue:
Ligand MSE 68 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3112
Unit cell:
a: 167.183Å b: 167.183Å c: 239.087Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.177 0.19
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

Broadening the phenotype and genotype spectrum of novel mutations in pontocerebellar hypoplasia with a comprehensive molecular literature review.
Ghasemi et al. (2024)

1 mention without citation

Structural basis for the tRNA-dependent activation of the terminal complex of selenocysteine synthesis in humans.
Puppala et al. (2023)