› 8gaj

X-ray diffraction
2.43Å resolution

Crystal Structure of E. coli LptA in complex with Podisus maculiventris Thanatin

Released:
DOI: 10.2210/pdb8gaj/pdb
PDB entry 8gaj coloured by chain, front view.
PDB entry 8gaj coloured by chain, side view.
PDB entry 8gaj coloured by chain, top view.
Source organisms:
Primary publication:
Discovery, characterization, and redesign of potent antimicrobial thanatin orthologs from Chinavia ubica and Murgantia histrionica targeting E. coli LptA.
Huynh K, Kibrom A, Donald BR, Zhou P
OpenAccess logo J Struct Biol X 8 100091 (2023)
PMID: 37416832

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238705 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lipopolysaccharide export system protein LptA Chains: A, C
Molecule details ›
Chains: A, C
Length: 132 amino acids
Theoretical weight: 14.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ADV1 (Residues: 28-159; Coverage: 84%)
Gene names: JW3167, b3200, lptA, yhbN
Sequence domains: LptA/(LptD N-terminal domain) LPS transport protein
Thanatin Chains: B, D
Molecule details ›
Chains: B, D
Length: 21 amino acids
Theoretical weight: 2.44 KDa
Source organism: Podisus maculiventris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55788 (Residues: 1-21; Coverage: 100%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 44.95Å b: 61.83Å c: 150.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links