› 8gam

Electron Microscopy
3.46Å resolution

Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications

Released:
DOI: 10.2210/pdb8gam/pdb
PDB entry 8gam coloured by chain, front view.
PDB entry 8gam coloured by chain, side view.
PDB entry 8gam coloured by chain, top view.
Source organism: Neisseria lactamica
Primary publication:
Exploiting activation and inactivation mechanisms in type I-C CRISPR-Cas3 for genome-editing applications.
Hu C, Myers MT, Zhou X, Hou Z, Lozen ML, Nam KH, Zhang Y, Ke A
Mol Cell 84 463-475.e5 (2024)
PMID: 38242128
Related structures: EMD-29900

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-270140 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (7 distinct):
CRISPR-associated protein, Csd2 family Chains: A, B, C, D, E, F, M
Molecule details ›
Chains: A, B, C, D, E, F, M
Length: 283 amino acids
Theoretical weight: 32.21 KDa
Source organism: Neisseria lactamica
Expression system: Escherichia coli
UniProt:
  • Canonical: D0W8X6 (Residues: 2-283; Coverage: 100%)
Gene name: NEILACOT_03980
Sequence domains: CRISPR-associated protein Cas7
CRISPR-associated protein, Csd1 family Chains: G, H
Molecule details ›
Chains: G, H
Length: 582 amino acids
Theoretical weight: 64.72 KDa
Source organism: Neisseria lactamica
Expression system: Escherichia coli B
UniProt:
  • Canonical: D0W8X5 (Residues: 1-582; Coverage: 100%)
Gene names: NEILACOT_03979, csd1
Sequence domains: CRISPR-associated protein (Cas_Csd1)
CRISPR-associated protein, Csd1 family Chains: I, J
Molecule details ›
Chains: I, J
Length: 124 amino acids
Theoretical weight: 14.25 KDa
Source organism: Neisseria lactamica
Expression system: Escherichia coli
UniProt:
  • Canonical: D0W8X5 (Residues: 459-582; Coverage: 21%)
Gene names: NEILACOT_03979, csd1
pre-crRNA processing endonuclease Chain: N
Molecule details ›
Chain: N
Length: 205 amino acids
Theoretical weight: 23.87 KDa
Source organism: Neisseria lactamica
Expression system: Escherichia coli
UniProt:
  • Canonical: D0W8X4 (Residues: 2-206; Coverage: 96%)
Gene names: NEILACOT_03978, cas5d
Sequence domains: CRISPR-associated protein (Cas_Cas5)
Target strand DNA (25-MER) Chain: L
Molecule details ›
Chain: L
Length: 25 nucleotides
Theoretical weight: 7.66 KDa
Non target strand DNA (5'-D(P*AP*TP*GP*AP*AP*CP*TP*TP*CP*AP*AP*AP*A)-3') Chain: O
Molecule details ›
Chain: O
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
crRNA (43-MER) Chain: K
Molecule details ›
Chain: K
Length: 43 nucleotides
Theoretical weight: 13.82 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.46Å
Relevant EMDB volumes: EMD-29900
Expression systems:
  • Escherichia coli
  • Escherichia coli B

Quick links

Citations

1 citations in other articles

Development and implementation of a Type I-C CRISPR-based programmable repression system for Neisseria gonorrhoeae.
Geslewitz et al. (2024)