8gan

Electron Microscopy
3.26Å resolution

Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-270137 (preferred)
Entry contents:
4 distinct polypeptide molecules
3 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
CRISPR-associated protein, Csd2 family Chains: A, B, C, D, E, F, M
Molecule details ›
Chains: A, B, C, D, E, F, M
Length: 283 amino acids
Theoretical weight: 32.21 KDa
Source organism: Neisseria lactamica
Expression system: Escherichia coli #1/H766
UniProt:
  • Canonical: D0W8X6 (Residues: 2-283; Coverage: 100%)
Gene name: NEILACOT_03980
Sequence domains: CRISPR-associated protein Cas7
CRISPR-associated protein, Csd1 family Chains: G, I, J
Molecule details ›
Chains: G, I, J
Length: 124 amino acids
Theoretical weight: 14.25 KDa
Source organism: Neisseria lactamica
Expression system: Escherichia coli
UniProt:
  • Canonical: D0W8X5 (Residues: 459-582; Coverage: 21%)
Gene names: NEILACOT_03979, csd1
CRISPR-associated protein, Csd1 family Chain: H
pre-crRNA processing endonuclease Chain: N
Target strand DNA (53-MER) Chain: L
Proximal Non target strand DNA (5'-D(P*AP*TP*GP*AP*AP*CP*TP*TP*CP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chain: O
Distal Non target strand DNA (5'-D(P*AP*TP*TP*AP*TP*AP*TP*TP*AP*AP*TP*AP*TP*TP*AP*TP*AP*TP*TP*TP*A)-3') Chain: Q
crRNA (43-MER) Chain: K

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.26Å
Relevant EMDB volumes: EMD-29901
Expression systems:
  • Escherichia coli #1/H766
  • Escherichia coli