› 8gao

Electron Microscopy
4.1Å resolution

bacteriophage T4 stalled primosome with mutant gp41-E227Q

Released:
DOI: 10.2210/pdb8gao/pdb
PDB entry 8gao coloured by chain, front view.
PDB entry 8gao coloured by chain, side view.
PDB entry 8gao coloured by chain, top view.
Source organism: Escherichia phage T4
Primary publication:
Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
Feng X, Spiering MM, de Luna Almeida Santos R, Benkovic SJ, Li H
Nat Commun 14 4396 (2023)
PMID: 37474605
Related structures: EMD-29902

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217234 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
DnaB-like replicative helicase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 432 amino acids
Theoretical weight: 48.8 KDa
Source organism: Escherichia phage T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04530 (Residues: 1-432; Coverage: 91%)
Gene name: 41
Sequence domains: DnaB-like helicase C terminal domain
DNA primase Chain: G
Molecule details ›
Chain: G
Length: 339 amino acids
Theoretical weight: 39.51 KDa
Source organism: Escherichia phage T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04520 (Residues: 3-341; Coverage: 99%)
Gene names: 58, 61
DNA (70-mer) Chain: M
DNA (70-mer) Chain: T
RNA (5'-D(*(GTP))-R(P*CP*CP*GP*A)-3') Chain: P

Ligands and Environments

3 bound ligands:
Ligand AGS 5 x AGS
Ligand MG 5 x MG
Ligand ZN 1 x ZN
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-29902
Expression system: Escherichia coli BL21(DE3)

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