› 8gb3

Electron Microscopy
3.7Å resolution

Structure of the Mycobacterium tuberculosis Hsp70 protein DnaK bound to the nucleotide exchange factor GrpE

Released:
DOI: 10.2210/pdb8gb3/pdb
PDB entry 8gb3 coloured by chain, front view.
PDB entry 8gb3 coloured by chain, side view.
PDB entry 8gb3 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structure of the M. tuberculosis DnaK-GrpE complex reveals how key DnaK roles are controlled.
Xiao X, Fay A, Molina PS, Kovach A, Glickman MS, Li H
OpenAccess logo Nat Commun 15 660 (2024)
PMID: 38253530
Related structures: EMD-29912

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243774 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein DnaK Chain: D
Molecule details ›
Chain: D
Length: 625 amino acids
Theoretical weight: 66.91 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WMJ9 (Residues: 1-625; Coverage: 100%)
Gene names: MTCY13E10.10, Rv0350, dnaK
Sequence domains: Hsp70 protein
Protein GrpE Chains: E, F
Molecule details ›
Chains: E, F
Length: 235 amino acids
Theoretical weight: 24.56 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WMT5 (Residues: 1-235; Coverage: 100%)
Gene names: MTCY13E10.11, Rv0351, grpE
Sequence domains: GrpE

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-29912
Expression system: Escherichia coli

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