› 8gfl

X-ray diffraction
2.36Å resolution

Crystal structure of soluble lytic transglycosylase Cj0843 of Campylobacter jejuni in complex with 4-Nitrophenyl N,N' diacetyl-beta-D-chitobioside inhibitor

Released:
DOI: 10.2210/pdb8gfl/pdb
PDB entry 8gfl coloured by chain, front view.
PDB entry 8gfl coloured by chain, side view.
PDB entry 8gfl coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Exploring the inhibition of the soluble lytic transglycosylase Cj0843c of Campylobacter jejuni via targeting different sites with different scaffolds.
Kumar V, Boorman J, Greenlee WJ, Zeng X, Lin J, van den Akker F
OpenAccess logo Protein Sci e4683 (2023)
PMID: 37209283

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lytic transglycosylase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 544 amino acids
Theoretical weight: 63.64 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand ZIE 1 x ZIE
Ligand CIT 1 x CIT
Ligand DMS 3 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: I23
Unit cell:
a: 177.067Å b: 177.067Å c: 177.067Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.206
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structural insights into peptidoglycan glycosidase EtgA binding to the inner rod protein EscI of the type III secretion system via a designed EscI-EtgA fusion protein.
Boorman et al. (2024)

1 mention without citation

Exploring the inhibition of the soluble lytic transglycosylase Cj0843c of Campylobacter jejuni via targeting different sites with different scaffolds.
Kumar et al. (2023)