› 8gfw

Electron Microscopy
3Å resolution

CryoEM structure of beta-2-adrenergic receptor in complex with GTP-bound Gs heterotrimer (transition intermediate #2 of 20)

Released:
DOI: 10.2210/pdb8gfw/pdb
PDB entry 8gfw coloured by chain, front view.
PDB entry 8gfw coloured by chain, side view.
PDB entry 8gfw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Time-resolved cryo-EM of G-protein activation by a GPCR.
Papasergi-Scott MM, Pérez-Hernández G, Batebi H, Gao Y, Eskici G, Seven AB, Panova O, Hilger D, Casiraghi M, He F, Maul L, Gmeiner P, Kobilka BK, Hildebrand PW, Skiniotis G
Nature (2024)
PMID: 38480881
Related structures: EMD-29986

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-272389 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.33 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
  • Best match: P63092-2 (Residues: 1-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 343 amino acids
Theoretical weight: 37.57 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Beta-2 adrenergic receptor Chain: R
Molecule details ›
Chain: R
Length: 460 amino acids
Theoretical weight: 51.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07550 (Residues: 1-24, 25-365, 366-413; Coverage: 100%)
Gene names: ADRB2, ADRB2R, B2AR
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

2 bound ligands:
Ligand GTP 1 x GTP
Ligand G1I 1 x G1I
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-29986
Expression systems:
  • Trichoplusia ni
  • Spodoptera frugiperda

Quick links

Citations

4 review citations

Perspectives on Ligand/Protein Binding Kinetics Simulations: Force Fields, Machine Learning, Sampling, and User-Friendliness.
Conflitti et al. (2023)
3 more