› 8ghe

Electron Microscopy
2.05Å resolution

The structure of h12-LOX in tetrameric form bound to endogenous inhibitor oleoyl-CoA

Released:
DOI: 10.2210/pdb8ghe/pdb
PDB entry 8ghe coloured by chain, front view.
PDB entry 8ghe coloured by chain, side view.
PDB entry 8ghe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of human arachidonate 12S-Lipoxygenase (12-LOX) bound to endogenous and exogenous inhibitors.
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal D, Glukhova A
Blood (2023)
PMID: 37506345
Related structures: EMD-40042

Function and Biology Details

Reactions catalysed: 
Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
Arachidonate + O(2) = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyunsaturated fatty acid lipoxygenase ALOX12 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 669 amino acids
Theoretical weight: 76.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P18054 (Residues: 2-663; Coverage: 100%)
Gene names: 12LO, ALOX12, LOG12
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FE2 4 x FE2
Ligand 3VV 1 x 3VV
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.05Å
Relevant EMDB volumes: EMD-40042
Expression system: Homo sapiens

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Citations

1 review citation

Antiplatelet strategies: past, present, and future.
Stanger et al. (2023)
2 other articles