› 8gis

X-ray diffraction
2.46Å resolution

Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.5mM Mn2+

Released:
DOI: 10.2210/pdb8gis/pdb
PDB entry 8gis coloured by chain, front view.
PDB entry 8gis coloured by chain, side view.
PDB entry 8gis coloured by chain, top view.
Source organisms:
Primary publication:
The structural basis for 2'-5'/3'-5'-cGAMP synthesis by cGAS.
Wu S, Gabelli SB, Sohn J
OpenAccess logo Nat Commun 15 4012 (2024)
PMID: 38740774

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-512808 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cyclic GMP-AMP synthase Chains: A, C
Molecule details ›
Chains: A, C
Length: 364 amino acids
Theoretical weight: 42.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8C6L5 (Residues: 147-507; Coverage: 71%)
Gene names: Cgas, Mb21d1
Sequence domains:
Palindromic DNA18 Chains: E, F, I, J
Molecule details ›
Chains: E, F, I, J
Length: 18 nucleotides
Theoretical weight: 5.51 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ATP 2 x ATP
Ligand ZN 2 x ZN
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-2
Spacegroup: P212121
Unit cell:
a: 78.056Å b: 98.218Å c: 142.465Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.23
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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