› 8giz

Electron Microscopy
2.7Å resolution

E. coli clamp loader with open clamp

Released:
DOI: 10.2210/pdb8giz/pdb
PDB entry 8giz coloured by chain, front view.
PDB entry 8giz coloured by chain, side view.
PDB entry 8giz coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Oakley AJ, Xu Z-Q, Dixon NE
Related structures: EMD-40080

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-242274 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
DNA polymerase III subunit delta Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 38.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P28630 (Residues: 1-343; Coverage: 100%)
Gene names: JW0635, b0640, holA
Sequence domains:
DNA polymerase III subunit tau Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 431 amino acids
Theoretical weight: 47.6 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P06710 (Residues: 1-430; Coverage: 67%)
Gene names: JW0459, b0470, dnaX, dnaZ, dnaZX
Sequence domains:
DNA polymerase III subunit delta' Chain: E
Molecule details ›
Chain: E
Length: 334 amino acids
Theoretical weight: 36.98 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P28631 (Residues: 1-334; Coverage: 100%)
Gene names: JW1085, b1099, holB
Sequence domains:
DNA polymerase III subunit psi Chain: F
Molecule details ›
Chain: F
Length: 137 amino acids
Theoretical weight: 15.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P28632 (Residues: 1-137; Coverage: 100%)
Gene names: JW4334, b4372, holD
Sequence domains: DNA polymerase III psi subunit
Beta sliding clamp Chains: H, I
Molecule details ›
Chains: H, I
Length: 366 amino acids
Theoretical weight: 40.63 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand AGS 3 x AGS
Ligand MG 3 x MG
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.7Å
Relevant EMDB volumes: EMD-40080
Expression system: Escherichia coli BL21

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