8gju

X-ray diffraction
2.79Å resolution

Crystal structure of human methylmalonyl-CoA mutase (MMUT) in complex with methylmalonic acidemia type A protein (MMAA), coenzyme A, and GDP

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
(R)-methylmalonyl-CoA = succinyl-CoA
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-273371 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methylmalonic aciduria type A protein, mitochondrial Chains: A, B, D, F
Molecule details ›
Chains: A, B, D, F
Length: 349 amino acids
Theoretical weight: 38.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IVH4 (Residues: 72-418; Coverage: 83%)
Gene name: MMAA
Sequence domains: Methylmalonyl Co-A mutase-associated GTPase MeaB
Methylmalonyl-CoA mutase, mitochondrial Chains: H, J, K, L
Molecule details ›
Chains: H, J, K, L
Length: 748 amino acids
Theoretical weight: 83.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22033 (Residues: 11-750; Coverage: 99%)
Gene names: MMUT, MUT
Sequence domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 95.806Å b: 221.872Å c: 121.824Å
α: 90° β: 105.53° γ: 90°
R-values:
R R work R free
0.232 0.229 0.276
Expression system: Escherichia coli BL21(DE3)