› 8go3

Electron Microscopy
3.09Å resolution

Cryo-EM structure of Escherichia coli cytochrome bo3 in DDM detergent

Released:
DOI: 10.2210/pdb8go3/pdb
PDB entry 8go3 coloured by chain, front view.
PDB entry 8go3 coloured by chain, side view.
PDB entry 8go3 coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Cao HY, Li K, Li CY
Related structures: EMD-34171

Function and Biology Details

Reaction catalysed: 
2 ubiquinol + O(2) + n H(+)(Side 1) = 2 ubiquinone + 2 H(2)O + n H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142171 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cytochrome bo(3) ubiquinol oxidase subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 663 amino acids
Theoretical weight: 74.42 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0ABI8 (Residues: 1-663; Coverage: 100%)
Gene names: JW0421, b0431, cyoB
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Cytochrome bo(3) ubiquinol oxidase subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 315 amino acids
Theoretical weight: 34.95 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0ABJ1 (Residues: 1-315; Coverage: 100%)
Gene names: JW0422, b0432, cyoA
Sequence domains:
Cytochrome bo(3) ubiquinol oxidase subunit 3 Chain: C
Molecule details ›
Chain: C
Length: 204 amino acids
Theoretical weight: 22.64 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0ABJ3 (Residues: 1-204; Coverage: 100%)
Gene names: JW0420, b0430, cyoC
Sequence domains: Cytochrome c oxidase subunit III
Cytochrome bo(3) ubiquinol oxidase subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 109 amino acids
Theoretical weight: 12.04 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0ABJ6 (Residues: 1-109; Coverage: 100%)
Gene names: JW0419, b0429, cyoD
Sequence domains: Prokaryotic Cytochrome C oxidase subunit IV

Ligands and Environments

5 bound ligands:
Ligand HEM 1 x HEM
Ligand HEO 1 x HEO
Ligand CU 1 x CU
Ligand UQ8 1 x UQ8
Ligand 3PE 2 x 3PE
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.09Å
Relevant EMDB volumes: EMD-34171

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