› 8gs1

X-ray diffraction
2.7Å resolution

Crystal structure of AziU2-U3 complex from Streptomyces sahachiroi NRRL2485

Released:
DOI: 10.2210/pdb8gs1/pdb
PDB entry 8gs1 coloured by chain, front view.
PDB entry 8gs1 coloured by chain, side view.
PDB entry 8gs1 coloured by chain, top view.
Source organism: Streptomyces sahachiroi
Primary publication:
Oxidase Heterotetramer Completes 1-Azabicyclo[3.1.0]hexane Formation with the Association of a Nonribosomal Peptide Synthetase.
Cheng Y, Yi X, Zhang Y, He Q, Chen D, Cao W, Fang P, Liu W
J Am Chem Soc (2023)
PMID: 37043819

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-228025 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Azi28 Chains: A, C
Molecule details ›
Chains: A, C
Length: 221 amino acids
Theoretical weight: 24.09 KDa
Source organism: Streptomyces sahachiroi
Expression system: Escherichia coli
UniProt:
  • Canonical: B4XYC0 (Residues: 1-221; Coverage: 100%)
Gene name: azi28
Butirosin biosynthesis protein H N-terminal domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 345 amino acids
Theoretical weight: 38.32 KDa
Source organism: Streptomyces sahachiroi
Expression system: Escherichia coli
UniProt:
  • Canonical: B4XYC1 (Residues: 1-337; Coverage: 100%)
Gene name: azi29
Sequence domains: Butirosin biosynthesis protein H, N-terminal

Ligands and Environments

2 bound ligands:
Ligand FMT 21 x FMT
Ligand LYS 2 x LYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P6122
Unit cell:
a: 242.93Å b: 242.93Å c: 135.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.218
Expression system: Escherichia coli

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