8gst

X-ray diffraction
1.71Å resolution

Crystal structure of L-2,4-diketo-3-deoxyrhamnonate hydrolase from Sphingomonas sp. (pyruvate bound-form)

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-557375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,4-didehydro-3-deoxy-L-rhamnonate hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 296 amino acids
Theoretical weight: 32.34 KDa
Source organism: Sphingomonas sp. SKA58
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q1NEI7 (Residues: 2-285; Coverage: 100%)
Gene names: LRA6, SKA58_03585
Sequence domains: Fumarylacetoacetate (FAA) hydrolase family

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P1
Unit cell:
a: 64.254Å b: 66.228Å c: 75.256Å
α: 90.27° β: 91.43° γ: 105.2°
R-values:
R R work R free
0.181 0.18 0.218
Expression system: Escherichia coli DH5[alpha]