› 8gur

Electron Microscopy
2.84Å resolution

Cryo-EM structure of CP-CB2-G protein complex

Released:
DOI: 10.2210/pdb8gur/pdb
PDB entry 8gur coloured by chain, front view.
PDB entry 8gur coloured by chain, side view.
PDB entry 8gur coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of selective cannabinoid CB2 receptor activation.
Li X, Chang H, Bouma J, de Paus LV, Mukhopadhyay P, Paloczi J, Mustafa M, van der Horst C, Kumar SS, Wu L, Yu Y, van den Berg RJBHN, Janssen APA, Lichtman A, Liu ZJ, Pacher P, van der Stelt M, Heitman LH, Hua T
OpenAccess logo Nat Commun 14 1447 (2023)
PMID: 36922494
Related structures: EMD-34277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-273588 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Cannabinoid receptor 2 Chain: R
Molecule details ›
Chain: R
Length: 360 amino acids
Theoretical weight: 39.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P34972 (Residues: 1-360; Coverage: 100%)
Gene names: CB2A, CB2B, CNR2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
scFv16 Chain: S
Molecule details ›
Chain: S
Length: 259 amino acids
Theoretical weight: 27.78 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand 9GF 1 x 9GF
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.84Å
Relevant EMDB volumes: EMD-34277
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Goods and Bads of the Endocannabinoid System as a Therapeutic Target: Lessons Learned after 30 Years.
Maccarrone et al. (2023)
3 more

1 mention without citation

Structural and functional insights into the G protein-coupled receptors: CB1 and CB2.
Brust et al. (2023)