› 8gv7

X-ray diffraction
2.6Å resolution

Crystal structure of PN-SIA28 in complex with influenza hemagglutinin H18 A/flat-faced bat/Peru/033/2010

Released:
DOI: 10.2210/pdb8gv7/pdb
PDB entry 8gv7 coloured by chain, front view.
PDB entry 8gv7 coloured by chain, side view.
PDB entry 8gv7 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for a human broadly neutralizing influenza A hemagglutinin stem-specific antibody including H17/18 subtypes.
Chen Y, Wang F, Yin L, Jiang H, Lu X, Bi Y, Zhang W, Shi Y, Burioni R, Tong Z, Song H, Qi J, Gao GF
OpenAccess logo Nat Commun 13 7603 (2022)
PMID: 36494358

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-579575 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 36.41 KDa
Source organism: Influenza A virus (A/flat-faced bat/Peru/033/2010(H18N11))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: U5N1D3 (Residues: 15-339; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin HA2 chain Chain: B
Molecule details ›
Chain: B
Length: 176 amino acids
Theoretical weight: 20.12 KDa
Source organism: Influenza A virus (A/flat-faced bat/Peru/033/2010(H18N11))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: U5N1D3 (Residues: 340-515; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
PN-SIA28 heavy chain Chain: C
Molecule details ›
Chain: C
Length: 125 amino acids
Theoretical weight: 13.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
PN-SIA28 light chain Chain: D
Molecule details ›
Chain: D
Length: 108 amino acids
Theoretical weight: 11.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P63
Unit cell:
a: 140.427Å b: 140.427Å c: 195.734Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.222
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

2 review citations

Advances in Nucleic Acid Universal Influenza Vaccines.
Xu et al. (2024)
1 more