PDB 8gzu structure summary ‹ Protein Data Bank in Europe (PDBe)

Function and Biology Details

Reactions catalysed:

4 ferrocytochrome c + O(2) + 8 H(+)(Side 1) = 4 ferricytochrome c + 4 H(2)O(Side 2)

NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)

Succinate + a quinone = fumarate + a quinol

A UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + a lipid X = UDP + a lipid A disaccharide

Biochemical function:

oxidoreductase activity

Biological process:

proton transmembrane transport

Cellular component:

membrane

Structure analysis Details

Assembly composition:

hetero 436-mer (preferred)

PDBe Complex ID:

PDB-CPX-279099 (preferred)

Entry contents:

150 distinct polypeptide molecules

Macromolecules (150 distinct):

Transmembrane protein, putative Chains: 00, 55, A, a

Molecule details ›

Chains: 00, 55, A, a
Length: 490 amino acids
Theoretical weight: 57.81 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:

Canonical: Q22PJ5 (Residues: 1-490; Coverage: 100%)

Gene name: TTHERM_00361490

Protein phosphatase 2C, putative Chains: 01, 56, B, b

Molecule details ›

Chains: 01, 56, B, b
Length: 473 amino acids
Theoretical weight: 53.01 KDa
Source organism: Tetrahymena thermophila SB210
UniProt:

Canonical: Q22FX8 (Residues: 1-473; Coverage: 100%)

Gene name: TTHERM_00721790

COXTT3 Chains: 02, 57, C, c

SURF1-like protein Chains: 03, 58, D, d

TraB family protein Chains: 04, 59, E, e

Transmembrane protein, putative Chains: 05, 60, F, f

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, mitochondrial Chains: 06, 61, G, g

SURF1-like protein Chains: 07, 62, H, h

Transmembrane protein Chains: 08, 63, I, i

Transmembrane protein Chains: 09, 64, J, j

Annexin Chains: 0A, 45, 6T, 6t

Chromosome condensation regulator RCC1 repeat protein Chains: 0B, 46, BP, bp

Iron-binding zinc finger CDGSH type domain-containing protein Chains: 0C, 47, FS, fs

Phage protein Chains: 0D, 48, 4A, 4a

Ymf67 Chains: 0E, 49, Y7, y7

Ymf75 Chains: 0F, 50, Y5, y5

Ymf70 Chains: 0G, 51, Y0, y0

COXTT28 Chains: 0H, 52, Z1, z1

Unknown peptide Chains: 0I, 53, U1, u1

unknown peptide Chains: 0J, 54, QM, Qm, U2, qM, qm, u2

39S ribosomal protein L9, mitochondrial Chains: 10, 65, K, k

Transmembrane protein Chains: 11, 66, L, l

Transmembrane protein, putative Chains: 12, 67, M, m

Transmembrane protein, putative Chains: 13, 68, N, n

Mobilization protein Chains: 14, 69, O, o

YflT domain-containing protein Chains: 15, 70, P, p

Transmembrane protein, putative Chains: 16, 71, Q, q

Transmembrane protein Chains: 17, 72, R, r

Complex III subunit VII Chains: 18, 73, S, s

Transmembrane protein, putative Chains: 19, 74, T, t

NADH dehydrogenase subunit 1 Chains: 1B, 1b

Transmembrane protein, putative Chains: 20, 75, U, u

Transmembrane protein Chains: 21, 76, V, v

Transmembrane protein, putative Chains: 22, 77, W, w

Transmembrane protein, putative Chains: 23, 78, X, x

Lysozyme Chains: 24, 79, Y, y

Transmembrane protein Chains: 25, 80, Z, z

Cytochrome c oxidase subunit 1 Chains: 26, 81, C1, c1

Cytochrome c oxidase polypeptide II Chains: 27, 82, C2, c2

Ymf68 Chains: 28, 83, C3, c3

Cytochrome C oxidase subunit Vb protein Chains: 29, 84, VB, vb

NADH:quinone oxidoreductase/Mrp antiporter transmembrane domain-containing protein Chains: 2B, 2b

NmrA-like domain-containing protein Chains: 2E, 2e

Transmembrane protein, putative Chains: 2F, 2f

SDHTT3 Chains: 2G, 2g

Diphthamide synthesis protein Chains: 2H, 2h

DUF4885 domain-containing protein Chains: 2I, 2i

Transmembrane protein, putative Chains: 2J, 2j

Transmembrane protein, putative Chains: 2K, 2k

Transposase Chains: 2L, 2l

Transmembrane protein, putative Chains: 2M, 2m

Transmembrane protein, putative Chains: 2N, 2n

SDHTT11 Chains: 2O, 2o

Transmembrane protein, putative Chains: 30, 6A, 6a, 85

Structural protein Chains: 31, 6B, 6b, 86

Transmembrane protein, putative Chains: 32, 6C, 6c, 87

Decapping nuclease Chains: 33, 6L, 6l, 88

Transmembrane protein, putative Chains: 34, 7A, 7a, 89

Transmembrane protein Chains: 35, 7C, 7c, 90

CTF/NF-I domain-containing protein Chains: 36, 7L, 7l, 91

Oxoglutarate/malate translocator protein, putative Chains: 37, 92, M1, m1

2-oxoglutarate/malate carrier protein Chains: 38, 93, M2, m2

Carrier protein Chains: 39, 94, M3, m3

Mitochondrial import inner membrane translocase subunit Chains: 1T, 1t, 40, 95

Tim10/DDP family zinc finger protein Chains: 2T, 2t, 41, 96

Tim10/DDP family zinc finger protein Chains: 3T, 3t, 42, 97

Transposase Chains: 43, 4T, 4t, 98

Cullin domain-containing protein Chains: 44, 5T, 5t, 99

Ymf58 Chains: 4L, 4l

Ymf57 Chains: 5B, 5b

Transmembrane protein, putative Chains: A1, a1

Ribosomal protein/NADH dehydrogenase domain-containing protein Chains: A2, a2

Transmembrane protein, putative Chains: A3, a3

ETC complex I subunit motif protein Chains: A5, a5

NADH dehydrogenase, putative Chains: A6, a6

37S ribosomal protein S25, mitochondrial Chains: A7, a7

Transmembrane protein Chains: A8, a8

NAD-dependent epimerase/dehydratase domain-containing protein Chains: A9, a9

Acyl carrier protein Chains: AB, ab

Acyl carrier protein Chains: AC, ac

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Chains: AL, al

Transmembrane protein Chains: AM, am

Transmembrane protein, putative Chains: AN, an

Transmembrane protein Chains: B2, b2

Transmembrane protein, putative Chains: B3, b3

NDUB4 Chains: B4, b4

Transmembrane protein Chains: B6, b6

CHCH domain-containing protein Chains: B7, b7

Transmembrane protein Chains: B8, b8

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Chains: B9, b9

NDUB10 Chains: BL, bl

Transmembrane protein, putative Chains: BM, bm

Transmembrane protein Chains: C4, c4

2Fe-2S ferredoxin-type domain-containing protein Chains: FX, fx

Gamma-carbonic anhydrase Chains: G1, g1

Gamma-carbonic anhydrase Chains: G2, g2

Transcription factor apfi protein, putative Chains: G3, g3

J domain-containing protein Chains: J1, j1

NADH-ubiquinone oxidoreductase chain 1 Chains: N1, n1

NADH:quinone oxidoreductase/Mrp antiporter transmembrane domain-containing protein Chains: N2, n2

NADH-ubiquinone oxidoreductase chain 3 Chains: N3, n3

NADH-ubiquinone oxidoreductase chain 4 Chains: N4, n4

NADH:quinone oxidoreductase/Mrp antiporter transmembrane domain-containing protein Chains: N5, n5

Ymf62 Chains: N6, n6

Transmembrane protein, putative Chains: P1, p1

NDUPH2 Chains: P2, p2

Peptidase M16 C-terminal domain-containing protein Chains: QA, Qa, qA, qa

Peptidase M16 inactive domain protein Chains: QB, Qb, qB, qb

Cytochrome b/b6 N-terminal region profile domain-containing protein Chains: QC, Qc, qC, qc

Cytochrome protein c1 Chains: QD, Qd, qD, qd

Rieske domain-containing protein Chains: QE, Qe, qE, qe

Ubiquinol-cytochrome C reductase hinge domain-containing protein Chains: QF, Qf, qF, qf

Sulphotransf domain-containing protein Chains: QG, Qg, qG, qg

Transmembrane protein, putative Chains: QH, Qh, qH, qh

Transmembrane protein, putative Chains: QI, Qi, qI, qi

Transmembrane protein, putative Chains: QJ, Qj, qJ, qj

UQCRTT2 Chains: QL, Ql, qL, ql

NADH-ubiquinone oxidoreductase 75 kDa subunit Chains: S1, s1

NADH-quinone oxidoreductase subunit D domain-containing protein Chains: S2, s2

NADH:ubiquinone oxidoreductase 30kDa subunit domain-containing protein Chains: S3, s3

NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Chains: S4, s4

GRAM domain protein Chains: S5, s5

Zinc finger CHCC-type domain-containing protein Chains: S6, s6

NADH:ubiquinone oxidoreductase-like 20kDa subunit domain-containing protein Chains: S7, s7

4Fe-4S ferredoxin-type domain-containing protein Chains: S8, s8

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial Chains: SA, sa

succinate dehydrogenase Chains: SB, sb

Transmembrane protein Chains: SC, sc

SDHD Chains: SD, sd

lipid-A-disaccharide synthase Chains: T1, t1

AMP-dependent synthetase/ligase domain-containing protein Chains: T2, t2

RNase III domain-containing protein Chains: T3, t3

Transmembrane protein Chains: T4, t4

Transmembrane protein, putative Chains: T5, t5

COX assembly mitochondrial protein Chains: T6, t6

Transmembrane protein, putative Chains: T7, t7

Transmembrane protein Chains: T8, t8

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Chains: T9, t9

Transmembrane protein Chains: TA, ta

Transmembrane protein, putative Chains: TB, tb

Transmembrane protein Chains: TC, tc

Transmembrane protein, putative Chains: TD, td

Transmembrane protein, putative Chains: TE, te

NDUTT15 Chains: TF, tf

NDUTT16 Chains: TG, tg

NDUTT17 Chains: TH, th

Thioredoxin domain-containing protein Chains: TX, tx

NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Chains: V1, v1

NADH-ubiquinone oxidoreductase 24 kDa subunit Chains: V2, v2

NADH-ubiquinone oxidoreductase 21kDa subunit N-terminal domain-containing protein Chains: X1, x1

Ligands and Environments

8 bound ligands:

2 modified residues:

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.

Resolution: 4.18Å

Relevant EMDB volumes: EMD-34403

Protein Data Bank in Europe

Cryo-EM structure of Tetrahymena thermophila respiratory Megacomplex MC (IV2+I+III2+II)2

Function and Biology Details

Structure analysis Details

Ligands and Environments

Experiments and Validation Details

Quick links

Citations

4 citation in other articles

PDBe › 8gzu

Cryo-EM structure of Tetrahymena thermophila respiratory Megacomplex MC (IV2+I+III2+II)2

Function and Biology Details

Structure analysis Details

Ligands and Environments

Experiments and Validation Details

Quick links

Citations

4 citation in other articles