› 8h0d

X-ray diffraction
2.01Å resolution

Structure of the thermolabile hemolysin from Vibrio alginolyticus (in complex with docosahexaenoic acid)

Released:
DOI: 10.2210/pdb8h0d/pdb
PDB entry 8h0d coloured by chain, front view.
PDB entry 8h0d coloured by chain, side view.
PDB entry 8h0d coloured by chain, top view.
Source organism: Vibrio alginolyticus
Primary publication:
Catalytic site flexibility facilitates the substrate and catalytic promiscuity of Vibrio dual lipase/transferase.
Wang C, Liu C, Zhu X, Peng Q, Ma Q
OpenAccess logo Nat Commun 14 4795 (2023)
PMID: 37558668

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermolabile hemolysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 426 amino acids
Theoretical weight: 48.38 KDa
Source organism: Vibrio alginolyticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A7Y4B3E8 (Residues: 1-418; Coverage: 100%)
Gene names: F0254_14065, GHY86_02245
Sequence domains: GDSL-like Lipase/Acylhydrolase

Ligands and Environments

3 bound ligands:
Ligand HXA 1 x HXA
Ligand 1PS 2 x 1PS
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 65.671Å b: 72.13Å c: 83.489Å
α: 90° β: 101.61° γ: 90°
R-values:
R R work R free
0.193 0.191 0.225
Expression system: Escherichia coli BL21(DE3)

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