› 8h0r

X-ray diffraction
1.2Å resolution

Crystal structure of a cataract-causing crystallin mutant (mouse CRYBB1 Y202X)

Released:
DOI: 10.2210/pdb8h0r/pdb
PDB entry 8h0r coloured by chain, front view.
PDB entry 8h0r coloured by chain, side view.
PDB entry 8h0r coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Cataract-causing Y204X mutation of crystallin protein CRYβB1 promotes its C-terminal degradation and higher-order oligomerization.
Jing X, Zhu M, Lu X, Wei P, Shi L, Zhang BY, Xu Y, Tang YP, Xiang DM, Gong P
OpenAccess logo J Biol Chem 299 104953 (2023)
PMID: 37356717

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-220978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-crystallin B1B Chains: A, B
Molecule details ›
Chains: A, B
Length: 91 amino acids
Theoretical weight: 10.76 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WVJ5 (Residues: 53-143; Coverage: 36%)
Gene name: Crybb1
Sequence domains: Beta/Gamma crystallin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 39.919Å b: 48.437Å c: 113.321Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.194
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Cataract-causing Y204X mutation of crystallin protein CRYβB1 promotes its C-terminal degradation and higher-order oligomerization.
Jing et al. (2023)