Function and Biology Details
Reaction catalysed:
S-adenosyl-L-methionine + tRNA containing 5-(aminomethyl)-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing ((5-methylamino)methyl)-2-thiouridylate
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
tRNA (mnm(5)s(2)U34)-methyltransferase (preferred)
PDBe Complex ID:
PDB-CPX-173449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (mnm(5)s(2)U34)-methyltransferase
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 21.96 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Putative rRNA methylase
Length: 195 amino acids
Theoretical weight: 21.96 KDa
Source organism: Staphylococcus aureus subsp. aureus NCTC 8325
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q2FXG9 (Residues: 1-187; Coverage: 100%)
Sequence domains: Putative rRNA methylase
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
PAL/PLS BEAMLINE 5C (4A)
Spacegroup:
P212121
Expression system: Escherichia coli BL21(DE3)
