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Near-atomic, non-icosahedrally averaged structure of giant virus Paramecium bursaria chlorella virus 1.

<div class='caption-title'>Overall structure of the PBCV-1 capsid.</div>
<div class='caption-title'>Structures of the MCP, MCP variants, penton protein, and penton protein variant.</div>
<div class='caption-title'>Structures of the pseudo-hexameric and pentameric capsomers.</div>
Shao Q, Agarkova IV, Noel EA, Dunigan DD, Liu Y, Wang A, Guo M, Xie L, Zhao X, Rossmann MG, Van Etten JL, Klose T, Fang Q
OpenAccess logo Nat Commun 13 6476 (2022)
Cited: 4 times
EuropePMC logo PMID: 36309542

Abstract

Giant viruses are a large group of viruses that infect many eukaryotes. Although components that do not obey the overall icosahedral symmetry of their capsids have been observed and found to play critical roles in the viral life cycles, identities and high-resolution structures of these components remain unknown. Here, by determining a near-atomic-resolution, five-fold averaged structure of Paramecium bursaria chlorella virus 1, we unexpectedly found the viral capsid possesses up to five major capsid protein variants and a penton protein variant. These variants create varied capsid microenvironments for the associations of fibers, a vesicle, and previously unresolved minor capsid proteins. Our structure reveals the identities and atomic models of the capsid components that do not obey the overall icosahedral symmetry and leads to a model for how these components are assembled and initiate capsid assembly, and this model might be applicable to many other giant viruses.

Reviews citing this publication (4)

  1. Kratosvirus quantuckense: the history and novelty of an algal bloom disrupting virus and a model for giant virus research. Truchon AR, Chase EE, Gann ER, Moniruzzaman M, Creasey BA, Aylward FO, Xiao C, Gobler CJ, Wilhelm SW. Front Microbiol 14 1284617 (2023)
  2. Biotechnologies for bulk production of microalgal biomass: from mass cultivation to dried biomass acquisition. Qin S, Wang K, Gao F, Ge B, Cui H, Li W. Biotechnol Biofuels Bioprod 16 131 (2023)
  3. Lessons from Chloroviruses: the Complex and Diverse Roles of Viruses in Food Webs. DeLong JP, Van Etten JL, Dunigan DD. J Virol 97 e0027523 (2023)
  4. Viruses of the Turriviridae: an emerging model system for studying archaeal virus-host interactions. Overton MS, Manuel RD, Lawrence CM, Snyder JC. Front Microbiol 14 1258997 (2023)


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