› 8h39

X-ray diffraction
2.01Å resolution

Structure of Acb2 complexed with c-di-AMP

Released:
DOI: 10.2210/pdb8h39/pdb
PDB entry 8h39 coloured by chain, front view.
PDB entry 8h39 coloured by chain, side view.
PDB entry 8h39 coloured by chain, top view.
Source organism: Pseudomonas phage PaP2
Primary publication:
Bacteriophages inhibit and evade cGAS-like immune function in bacteria.
Huiting E, Cao X, Ren J, Athukoralage JS, Luo Z, Silas S, An N, Carion H, Zhou Y, Fraser JS, Feng Y, Bondy-Denomy J
Cell (2023)
PMID: 36750095

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232316 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anti-CBASS protein 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 93 amino acids
Theoretical weight: 10.72 KDa
Source organism: Pseudomonas phage PaP2
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1C8HPQ3 (Residues: 1-93; Coverage: 100%)
Gene names: PaMx33_ORF24, acb2

Ligands and Environments

2 bound ligands:
Ligand 2BA 4 x 2BA
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: P321
Unit cell:
a: 104.142Å b: 104.142Å c: 102.043Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.208 0.231
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The highly diverse antiphage defence systems of bacteria.
Georjon et al. (2023)
6 more

1 mention without citation

Bacteriophages inhibit and evade cGAS-like immune function in bacteria.
Huiting et al. (2023)