› 8h3q

Electron Microscopy
3.76Å resolution

Cryo-EM Structure of the CAND1-Cul3-Rbx1 complex

Released:
DOI: 10.2210/pdb8h3q/pdb
PDB entry 8h3q coloured by chain, front view.
PDB entry 8h3q coloured by chain, side view.
PDB entry 8h3q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dynamic molecular architecture and substrate recruitment of cullin3-RING E3 ligase CRL3KBTBD2.
Hu Y, Zhang Z, Mao Q, Zhang X, Hao A, Xun Y, Wang Y, Han L, Zhan W, Liu Q, Yin Y, Peng C, Moresco EMY, Chen Z, Beutler B, Sun L
Nat Struct Mol Biol 31 336-350 (2024)
PMID: 38332366
Related structures: EMD-34473

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-231843 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cullin-associated NEDD8-dissociated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 1230 amino acids
Theoretical weight: 136.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86VP6 (Residues: 1-1230; Coverage: 100%)
Gene names: CAND1, KIAA0829, TIP120, TIP120A
Sequence domains:
Cullin-3 Chain: C
Molecule details ›
Chain: C
Length: 768 amino acids
Theoretical weight: 89.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13618 (Residues: 1-768; Coverage: 100%)
Gene names: CUL3, KIAA0617
Sequence domains:
E3 ubiquitin-protein ligase RBX1 Chain: E
Molecule details ›
Chain: E
Length: 108 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62877 (Residues: 1-108; Coverage: 100%)
Gene names: RBX1, RNF75, ROC1
Sequence domains: RING-H2 zinc finger domain

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.76Å
Relevant EMDB volumes: EMD-34473
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

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