› 8h40

Electron Microscopy
3.6Å resolution

Cryo-EM structure of the transcription activation complex NtcA-TAC

Released:
DOI: 10.2210/pdb8h40/pdb
PDB entry 8h40 coloured by chain, front view.
PDB entry 8h40 coloured by chain, side view.
PDB entry 8h40 coloured by chain, top view.
Source organism: Anabaena
Primary publication:
DNA looping mediates cooperative transcription activation.
Han SJ, Jiang YL, You LL, Shen LQ, Wu X, Yang F, Cui N, Kong WW, Sun H, Zhou K, Meng HC, Chen ZP, Chen Y, Zhang Y, Zhou CZ
Nat Struct Mol Biol (2024)
PMID: 38177666
Related structures: EMD-34476

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
PDBe Complex ID:
PDB-CPX-230657 (preferred)
Entry contents:
7 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (9 distinct):
DNA-directed RNA polymerase subunit beta Chain: A
Molecule details ›
Chain: A
Length: 1132 amino acids
Theoretical weight: 126.78 KDa
Source organism: Anabaena
Expression system: Escherichia coli
UniProt:
  • Canonical: P22703 (Residues: 2-1131; Coverage: 100%)
Gene names: alr1594, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: B
Molecule details ›
Chain: B
Length: 1350 amino acids
Theoretical weight: 147.14 KDa
Source organism: Anabaena
Expression system: Escherichia coli
UniProt:
  • Canonical: P22705 (Residues: 6-1355; Coverage: 100%)
Gene names: alr1596, rpoC2
Sequence domains:
DNA-directed RNA polymerase subunit alpha Chains: C, D
Molecule details ›
Chains: C, D
Length: 236 amino acids
Theoretical weight: 26.17 KDa
Source organism: Anabaena
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YPK3 (Residues: 2-235; Coverage: 74%)
Gene names: all4191, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit gamma Chain: E
Molecule details ›
Chain: E
Length: 625 amino acids
Theoretical weight: 70.65 KDa
Source organism: Anabaena
Expression system: Escherichia coli
UniProt:
  • Canonical: P22704 (Residues: 1-625; Coverage: 100%)
Gene names: alr1595, rpoC1
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: F
Molecule details ›
Chain: F
Length: 78 amino acids
Theoretical weight: 9.14 KDa
Source organism: Anabaena
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YNB9 (Residues: 1-78; Coverage: 100%)
Gene names: asr4648, rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor SigA Chain: G
Molecule details ›
Chain: G
Length: 390 amino acids
Theoretical weight: 45.72 KDa
Source organism: Anabaena
Expression system: Escherichia coli
UniProt:
  • Canonical: P26683 (Residues: 1-390; Coverage: 100%)
Gene names: all5263, rpoD, sigA
Sequence domains:
Global nitrogen regulator Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 223 amino acids
Theoretical weight: 24.95 KDa
Source organism: Anabaena
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A4U6 (Residues: 1-223; Coverage: 100%)
Gene names: alr4392, bifA, ntcA
Sequence domains:
DNA (125-MER) Chain: 1
Molecule details ›
Chain: 1
Length: 125 nucleotides
Theoretical weight: 38.72 KDa
DNA (125-MER) Chain: 2
Molecule details ›
Chain: 2
Length: 125 nucleotides
Theoretical weight: 38.57 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-34476
Expression system: Escherichia coli

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