› 8h4e

X-ray diffraction
2.06Å resolution

Blasnase-T13A/P55N with D-asn

Released:
DOI: 10.2210/pdb8h4e/pdb
PDB entry 8h4e coloured by chain, front view.
PDB entry 8h4e coloured by chain, side view.
PDB entry 8h4e coloured by chain, top view.
Source organism: Bacillus paralicheniformis
Entry authors: Lu F, Wang W, Chi H, Ran T

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-498336 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 35.46 KDa
Source organism: Bacillus paralicheniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6I7U6Y2 (Residues: 1-322; Coverage: 100%)
Gene names: B4121_3474, PVN32_05695
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand FMT 8 x FMT
Ligand DSG 2 x DSG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P41212
Unit cell:
a: 92.42Å b: 92.42Å c: 232.452Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.221
Expression system: Escherichia coli

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