› 8h4u

Electron Microscopy
3.5Å resolution

Cryo-EM structure of a riboendonuclease

Released:
DOI: 10.2210/pdb8h4u/pdb
PDB entry 8h4u coloured by chain, front view.
PDB entry 8h4u coloured by chain, side view.
PDB entry 8h4u coloured by chain, top view.
Source organism: Thermoclostridium caenicola
Primary publication:
Structural Basis for the Ribonuclease Activity of a Thermostable CRISPR-Cas13a from Thermoclostridium caenicola.
Wang F, Zhang C, Xu H, Zeng W, Ma L, Li Z
J Mol Biol 435 168197 (2023)
PMID: 37442412
Related structures: EMD-34484

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227507 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endoribonuclease Cas13a Chain: A
Molecule details ›
Chain: A
Length: 1225 amino acids
Theoretical weight: 143.58 KDa
Source organism: Thermoclostridium caenicola
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1M6GDI0 (Residues: 1-1225; Coverage: 100%)
Gene name: SAMN05444373_102315

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-34484
Expression system: Escherichia coli BL21(DE3)

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