› 8h59

X-ray diffraction
2.15Å resolution

A fungal MAP kinase in complex with an inhibitor

Released:
DOI: 10.2210/pdb8h59/pdb
PDB entry 8h59 coloured by chain, front view.
PDB entry 8h59 coloured by chain, side view.
PDB entry 8h59 coloured by chain, top view.
Source organism: Pyricularia oryzae 70-15
Primary publication:
Structure-Aided Identification of an Inhibitor Targets Mps1 for the Management of Plant-Pathogenic Fungi.
Kong Z, Zhang X, Zhou F, Tang L, Chen Y, Li S, Zhang X, Kuai L, Su W, Cui W, Cai J, Wang Y, Yang J, Peng YL, Wang D, Liu J
OpenAccess logo mBio e0288322 (2023)
PMID: 36779710

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase MPS1 Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 46.16 KDa
Source organism: Pyricularia oryzae 70-15
Expression system: Escherichia coli
UniProt:
  • Canonical: G4N374 (Residues: 5-412; Coverage: 98%)
Gene names: MGG_04943, MPS1
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand KR9 1 x KR9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P3221
Unit cell:
a: 75.04Å b: 75.04Å c: 159.058Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.203
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Characterization of PcSTT3B as a Key Oligosaccharyltransferase Subunit Involved in N-glycosylation and Its Role in Development and Pathogenicity of Phytophthora capsici.
Cui et al. (2023)

1 mention without citation

Structure-Aided Identification of an Inhibitor Targets Mps1 for the Management of Plant-Pathogenic Fungi.
Kong et al. (2023)