Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
SARS-CoV-2 primase complex (preferred)
PDBe Complex ID:
PDB-CPX-145081 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3C-like proteinase nsp5
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.77 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli
UniProt:
Sequence domains: Coronavirus endopeptidase C30
Length: 306 amino acids
Theoretical weight: 33.77 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli
UniProt:
- Canonical:
P0DTD1 (Residues: 3264-3569; Coverage: 4%)
Sequence domains: Coronavirus endopeptidase C30
3C-like proteinase nsp5
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.07 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: chemical production metagenome
UniProt:
Length: 10 amino acids
Theoretical weight: 1.07 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: chemical production metagenome
UniProt:
- Canonical: P0DTD1 (Residues: 3258-3267; Coverage: 0%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SSRF BEAMLINE BL02U1
Spacegroup:
C2
Expression systems:
- Escherichia coli
- chemical production metagenome
