› 8h7o

X-ray diffraction
1.55Å resolution

Crystal structure of aqualigase

Released:
DOI: 10.2210/pdb8h7o/pdb
PDB entry 8h7o coloured by chain, front view.
PDB entry 8h7o coloured by chain, side view.
PDB entry 8h7o coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Li H, Ma MZ, Zhang LJ, Dai L, Chen C-C, Guo R-T

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Subtilisin Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 28.36 KDa
Source organism: Bacillus subtilis
Expression system: Bacillus subtilis
UniProt:
  • Canonical: A0A7U5AV24 (Residues: 108-382; Coverage: 78%)
Gene name: KS08_04965
Sequence domains: Subtilase family

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL02U1
Spacegroup: P21
Unit cell:
a: 36.85Å b: 78.53Å c: 41.16Å
α: 90° β: 114.84° γ: 90°
R-values:
R R work R free
0.124 0.123 0.157
Expression system: Bacillus subtilis

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