› 8h8x

Electron Microscopy
3.92Å resolution

Cryo-EM structure of HACE1 monomer

Released:
DOI: 10.2210/pdb8h8x/pdb
PDB entry 8h8x coloured by chain, front view.
PDB entry 8h8x coloured by chain, side view.
PDB entry 8h8x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for the Enzymatic Activity of the HACE1 HECT-Type E3 Ligase Through N-Terminal Helix Dimerization.
Singh S, Machida S, Tulsian NK, Choong YK, Ng J, Shankar S, Liu Y, Chandiramani KV, Shi J, Sivaraman J
OpenAccess logo Adv Sci (Weinh) e2207672 (2023)
PMID: 37537642
Related structures: EMD-34551

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-219647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase HACE1 Chain: A
Molecule details ›
Chain: A
Length: 909 amino acids
Theoretical weight: 102.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8IYU2 (Residues: 1-909; Coverage: 100%)
Gene names: HACE1, KIAA1320
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.92Å
Relevant EMDB volumes: EMD-34551
Expression system: Escherichia coli BL21

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Citations

1 citations in other articles

Structural Basis for the Enzymatic Activity of the HACE1 HECT-Type E3 Ligase Through N-Terminal Helix Dimerization.
Singh et al. (2023)