› 8hbd

Electron Microscopy
2.99Å resolution

Cryo-EM structure of IRL1620-bound ETBR-Gi complex

Released:
DOI: 10.2210/pdb8hbd/pdb
PDB entry 8hbd coloured by chain, front view.
PDB entry 8hbd coloured by chain, side view.
PDB entry 8hbd coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of peptide recognition and activation of endothelin receptors.
Ji Y, Duan J, Yuan Q, He X, Yang G, Zhu S, Wu K, Hu W, Gao T, Cheng X, Jiang H, Eric Xu H, Jiang Y
Nat Commun 14 1268 (2023)
PMID: 36882417
Related structures: EMD-34619

Function and Biology Details

Reaction catalysed: 
Oplophorus luciferin + O(2) = oxidized Oplophorus luciferin + CO(2) + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-230905 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 40.45 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63096 (Residues: 1-354; Coverage: 100%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 377 amino acids
Theoretical weight: 41.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
scFv16 Chain: H
Molecule details ›
Chain: H
Length: 285 amino acids
Theoretical weight: 30.36 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
IRL1620 Chain: L
Molecule details ›
Chain: L
Length: 15 amino acids
Theoretical weight: 1.84 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Endothelin receptor type B Chain: R
Molecule details ›
Chain: R
Length: 603 amino acids
Theoretical weight: 67.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P24530 (Residues: 27-424; Coverage: 96%)
Gene names: EDNRB, ETRB
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.99Å
Relevant EMDB volumes: EMD-34619
Expression system: Spodoptera frugiperda

Quick links

Citations

1 mention without citation

Structural insights into endothelin receptor signalling.
Shihoya et al. (2023)