› 8hbh

Electron Microscopy
3.1Å resolution

Structure of human soluble guanylate cyclase in the NO-activated state at 3.1 angstrom

Released:
DOI: 10.2210/pdb8hbh/pdb
PDB entry 8hbh coloured by chain, front view.
PDB entry 8hbh coloured by chain, side view.
PDB entry 8hbh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
NO binds to the distal site of haem in the fully activated soluble guanylate cyclase.
Liu R, Kang Y, Chen L
Nitric Oxide 134-135 17-22 (2023)
PMID: 36972843
Related structures: EMD-34632

Function and Biology Details

Reaction catalysed: 
GTP = 3',5'-cyclic GMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169668 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanylate cyclase soluble subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 690 amino acids
Theoretical weight: 77.57 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02108 (Residues: 1-690; Coverage: 100%)
Gene names: GUC1A3, GUCSA3, GUCY1A1, GUCY1A3
Sequence domains:
Guanylate cyclase soluble subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 619 amino acids
Theoretical weight: 70.6 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q02153 (Residues: 1-619; Coverage: 100%)
Gene names: GUC1B3, GUCSB3, GUCY1B1, GUCY1B3
Sequence domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
3 bound ligands:
Ligand MG 2 x MG
Ligand G2P 1 x G2P
Ligand NO 1 x NO
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-34632
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Per-ARNT-Sim Domains in Nitric Oxide Signaling by Soluble Guanylyl Cyclase.
Montfort WR. (2024)