› 8hcx

Electron Microscopy
3.5Å resolution

Cryo-EM structure of Endothelin1-bound ETBR-Gq complex

Released:
DOI: 10.2210/pdb8hcx/pdb
PDB entry 8hcx coloured by chain, front view.
PDB entry 8hcx coloured by chain, side view.
PDB entry 8hcx coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of peptide recognition and activation of endothelin receptors.
Ji Y, Duan J, Yuan Q, He X, Yang G, Zhu S, Wu K, Hu W, Gao T, Cheng X, Jiang H, Eric Xu H, Jiang Y
OpenAccess logo Nat Commun 14 1268 (2023)
PMID: 36882417
Related structures: EMD-34667

Function and Biology Details

Reaction catalysed: 
Oplophorus luciferin + O(2) = oxidized Oplophorus luciferin + CO(2) + light
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-272326 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(q) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 377 amino acids
Theoretical weight: 41.06 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Endothelin receptor type B Chain: C
Endothelin-1 Chain: D
scFv16 Chain: E
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-34667
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Cryo-electron microscopy for GPCR research and drug discovery in endocrinology and metabolism.
Duan et al. (2024)
1 more

2 mentions without citation

Structural insights into endothelin receptor signalling.
Shihoya et al. (2023)
1 more