› 8hd7

Electron Microscopy
3.52Å resolution

The intermediate pre-Tet-S1 state of G264A mutated Tetrahymena group I intron with 6nt 3'/5'-exon and 2-aminopurine nucleoside

Released:
DOI: 10.2210/pdb8hd7/pdb
PDB entry 8hd7 coloured by chain, front view.
PDB entry 8hd7 coloured by chain, side view.
PDB entry 8hd7 coloured by chain, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Cryo-EM reveals dynamics of Tetrahymena group I intron self-splicing
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023)
Related structures: EMD-34671

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197394 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
The intermediate pre-Tet-S1 state molecule of co-transcriptional folded G264A mutant Tetrahymena group I intron with 6nt 3'/5'-exon and 2-aminopurine nucleoside Chain: N
Molecule details ›
Chain: N
Length: 419 nucleotides
Theoretical weight: 135.16 KDa
Source organism: Tetrahymena thermophila
Expression system: synthetic construct
Sequence domains: Group I catalytic intron

Ligands and Environments

2 bound ligands:
Ligand SPD 1 x SPD
Ligand MG 27 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.52Å
Relevant EMDB volumes: EMD-34671
Expression system: synthetic construct

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