› 8hde

X-ray diffraction
2.7Å resolution

Brucella melitensis 7-alpha-Hydroxysteroid Dehydrogenase mutant: 1-53 truncation/I258M

Released:
DOI: 10.2210/pdb8hde/pdb
PDB entry 8hde coloured by chain, front view.
PDB entry 8hde coloured by chain, side view.
PDB entry 8hde coloured by chain, top view.
Source organism: Brucella melitensis
Entry authors: Liu ZY, Zhang RZ

Function and Biology Details

Reaction catalysed: 
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + NAD(+) = 3-alpha,12-alpha-dihydroxy-7-oxo-5-beta-cholanate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234833 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
7-alpha-hydroxysteroid dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 25.77 KDa
Source organism: Brucella melitensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YIN7 (Residues: 54-304; Coverage: 83%)
Gene name: BMEI0406
Sequence domains: Enoyl-(Acyl carrier protein) reductase

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: P3121
Unit cell:
a: 98.26Å b: 98.26Å c: 92.456Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.175 0.267
Expression system: Escherichia coli

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