› 8he4

X-ray diffraction
1.93Å resolution

The structure of chitin deacetylase Pst_13661 from Puccinia striiformis f. sp. tritici

Released:
DOI: 10.2210/pdb8he4/pdb
PDB entry 8he4 coloured by chain, front view.
PDB entry 8he4 coloured by chain, side view.
PDB entry 8he4 coloured by chain, top view.
Source organism: Puccinia striiformis f. sp. tritici
Primary publication:
Inhibition of chitin deacetylases to attenuate plant fungal diseases.
Liu L, Xia Y, Li Y, Zhou Y, Su X, Yan X, Wang Y, Liu W, Cheng H, Wang Y, Yang Q
OpenAccess logo Nat Commun 14 3857 (2023)
PMID: 37385996

Function and Biology Details

Reaction catalysed: 
Chitin + H(2)O = chitosan + acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NodB homology domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 31.64 KDa
Source organism: Puccinia striiformis f. sp. tritici
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A2S4W2W0 (Residues: 647-920; Coverage: 30%)
Gene name: PSTT_01692
Sequence domains: Polysaccharide deacetylase

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand 8GK 1 x 8GK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17B1
Spacegroup: P212121
Unit cell:
a: 39.754Å b: 65.693Å c: 106.806Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.193
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Powerful cell wall biomass degradation enzymatic system from saprotrophic Aspergillus fumigatus.
Tong et al. (2024)
1 more

1 mention without citation

Inhibition of chitin deacetylases to attenuate plant fungal diseases.
Liu et al. (2023)