› 8hg1

Electron Microscopy
2.8Å resolution

The structure of MPXV polymerase holoenzyme in replicating state

Released:
DOI: 10.2210/pdb8hg1/pdb
PDB entry 8hg1 coloured by chain, front view.
PDB entry 8hg1 coloured by chain, side view.
PDB entry 8hg1 coloured by chain, top view.
Source organism: Monkeypox virus
Primary publication:
Structure of monkeypox virus DNA polymerase holoenzyme.
Peng Q, Xie Y, Kuai L, Wang H, Qi J, Gao GF, Shi Y
Science eade6360 (2022)
PMID: 36520947
Related structures: EMD-34731

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-222092 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 1031 amino acids
Theoretical weight: 119.94 KDa
Source organism: Monkeypox virus
Expression system: Spodoptera
UniProt:
  • Canonical: A0A7H0DN44 (Residues: 1-1006; Coverage: 100%)
Gene names: MPXVgp056, OPG071, POL
Sequence domains:
Uracil-DNA glycosylase Chain: B
Molecule details ›
Chain: B
Length: 218 amino acids
Theoretical weight: 25.11 KDa
Source organism: Monkeypox virus
Expression system: Spodoptera
UniProt:
  • Canonical: M1LL92 (Residues: 1-218; Coverage: 100%)
Gene names: MPXVgp099, OPG116, UNG
DNA polymerase processivity factor component OPG148 Chain: C
Molecule details ›
Chain: C
Length: 426 amino acids
Theoretical weight: 49.2 KDa
Source organism: Monkeypox virus
Expression system: Spodoptera
UniProt:
  • Canonical: A0A7H0DNC0 (Residues: 1-426; Coverage: 100%)
Gene names: MPXVgp132, OPG148
Sequence domains: Chordopoxvirus A20R protein
DNA (25-MER) Chain: P
Molecule details ›
Chain: P
Length: 25 nucleotides
Theoretical weight: 7.68 KDa
DNA (38-MER) Chain: T
Molecule details ›
Chain: T
Length: 38 nucleotides
Theoretical weight: 11.68 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand TTP 1 x TTP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-34731
Expression system: Spodoptera

Quick links

Citations

4 review citations

Human Monkeypox: A Comprehensive Overview of Epidemiology, Pathogenesis, Diagnosis, Treatment, and Prevention Strategies.
Martínez-Fernández et al. (2023)
3 more

4 mentions without citation

Using AlphaFold Predictions in Viral Research.
Gutnik et al. (2023)
3 more