8hhm

Electron Microscopy
3.08Å resolution

Cryo-EM structure of the Cas12m2-crRNA-target DNA ternary complex intermediate state

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-270497 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated DNA-binding protein Cas12m Chain: A
Molecule details ›
Chain: A
Length: 598 amino acids
Theoretical weight: 66.46 KDa
Source organism: Mycolicibacterium mucogenicum
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DXB1 (Residues: 1-596; Coverage: 100%)
Gene name: cas12m
DNA (36-MER) Chain: C
Molecule details ›
Chain: C
Length: 36 nucleotides
Theoretical weight: 10.98 KDa
Source organism: Mycolicibacterium mucogenicum
Expression system: Not provided
DNA (36-MER) Chain: D
RNA (56-MER) Chain: B

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.08Å
Relevant EMDB volumes: EMD-34804
Expression systems:
  • Escherichia coli
  • Not provided