› 8hig

X-ray diffraction
2.33Å resolution

Co-crystal structure of C-terminal DNA binding domain of Saccharopolyspora erythraea GlnR in complex with its cognate promoter DNA

Released:
DOI: 10.2210/pdb8hig/pdb
PDB entry 8hig coloured by chain, front view.
PDB entry 8hig coloured by chain, side view.
PDB entry 8hig coloured by chain, top view.
Source organism: Saccharopolyspora erythraea NRRL 2338
Entry authors: Lin W, Xu JC, Zhang Y

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235587 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 27.63 KDa
Source organism: Saccharopolyspora erythraea NRRL 2338
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4FQD5 (Residues: 1-256; Coverage: 100%)
Gene names: SACE_7101, glnR
Sequence domains:
DNA (5'-D(*AP*CP*GP*TP*AP*AP*CP*AP*TP*CP*GP*CP*GP*GP*TP*AP*AP*CP*AP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: Saccharopolyspora erythraea NRRL 2338
Expression system: Not provided
DNA (5'-D(*GP*TP*GP*TP*TP*AP*CP*CP*GP*CP*GP*AP*TP*GP*TP*TP*AP*CP*GP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.16 KDa
Source organism: Saccharopolyspora erythraea NRRL 2338
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P3121
Unit cell:
a: 46.279Å b: 46.279Å c: 366.859Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.265 0.259 0.325
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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