› 8hik

Electron Microscopy
3.72Å resolution

The TPP-bound BRIL-SLC19A1/Fab/Nb ternary complex

Released:
DOI: 10.2210/pdb8hik/pdb
PDB entry 8hik coloured by chain, front view.
PDB entry 8hik coloured by chain, side view.
PDB entry 8hik coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanism of substrate recognition by folate transporter SLC19A1.
Dang Y, Zhou D, Du X, Zhao H, Lee CH, Yang J, Wang Y, Qin C, Guo Z, Zhang Z
OpenAccess logo Cell Discov 8 141 (2022)
PMID: 36575193
Related structures: EMD-34819

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209544 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Reduced folate transporter; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 683 amino acids
Theoretical weight: 75.11 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
  • Canonical: P41440 (Residues: 24-591; Coverage: 96%)
Gene names: FLOT1, RFC1, SLC19A1, cybC
Sequence domains:
Anti-BRIL Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 260 amino acids
Theoretical weight: 27.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Anti-Fab nanobody Chain: N
Molecule details ›
Chain: N
Length: 153 amino acids
Theoretical weight: 16.67 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21
Anti-BRIL Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 235 amino acids
Theoretical weight: 25.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand TPP 1 x TPP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.72Å
Relevant EMDB volumes: EMD-34819
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21

Quick links

Citations

1 citations in other articles

The Impacts of Slc19a3 Deletion and Intestinal SLC19A3 Insertion on Thiamine Distribution and Brain Metabolism in the Mouse.
Wen et al. (2023)

1 mention without citation

How Cryo-EM Has Expanded Our Understanding of Membrane Transporters.
Baril et al. (2023)