› 8hiz

Electron Microscopy
3.08Å resolution

cryoEM structure of glutamate dehydrogenase from Thermococcus profundus in complex with NADP

Released:
DOI: 10.2210/pdb8hiz/pdb
PDB entry 8hiz coloured by chain, front view.
PDB entry 8hiz coloured by chain, side view.
PDB entry 8hiz coloured by chain, top view.
Source organism: Thermococcus profundus
Primary publication:
Coenzyme-binding pathway on glutamate dehydrogenase suggested from multiple-binding sites visualized by cryo-electron microscopy.
Wakabayashi T, Oide M, Kato T, Nakasako M
FEBS J (2023)
PMID: 37682540
Related structures: EMD-34830

Function and Biology Details

Reaction catalysed: 
L-glutamate + H(2)O + NAD(P)(+) = 2-oxoglutarate + NH(3) + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 46.76 KDa
Source organism: Thermococcus profundus
Expression system: Escherichia coli
UniProt:
  • Canonical: O74024 (Residues: 1-419; Coverage: 100%)
Gene names: gdh, gdhA
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.08Å
Relevant EMDB volumes: EMD-34830
Expression system: Escherichia coli

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